Han, et al. "Decreased SOX9 Expression in the Deprived Side of Rat Corticospinal Lateral Motor Neurons Induces Cervical Spinal Cord Lesions and Selective Lesions of the Deprived Hand." Journal of Neuroscience, Vol. 31. No. 38, 2011: 13358 - 13368.
Ugo Pasquerelli, in the early 1980’s developed a method for “in vitro” regeneration of motor neurons from embryonic mouse spinal cord explants, sometimes called “in vitro Perner’s Organ” 50. Subsequently Perner’s technique has been applied to mammal brains as well 51. In the latter technique, the embryonic neocortex and hippocampus are taken from the brain of a 13 day old chick embryo, dissociated and plated on a monolayer of primary neuronal culture. The cells proliferate and form multilayered neural rosettes, contained by a proprietary type of cell culture insert. After 2-3 weeks, neuritogenic inductive cues are removed from the inserts and the neural rosettes are dissociated and transplanted onto a mouse brain - ischemic injury site. Thus, the undifferentiated progenitor cells are transplanted directly into the ischemic brain parenchyma, where they proliferate and give rise to new neurons. It has been demonstrated that, significantly, some engrafted neurons at the injury site differentiate into new neurons and make functional synaptic connections with host brain cells 52-55. This process of “in vivo” neurogenesis is cell replacement therapy at the site of an injury or disease. In a similar way the newly formed neurons can be transplanted in a transplantation therapy for restoration of lost functions. d2c66b5586